Antibody conjugation
Purified antibodies are labeled on request either with enzymes (HRP and AP) or with fluorogens (FITC or PE). Optimized procedures guarantee preservation of immunological reactivity and enzymatic activity. Alternatively, antibodies are biotinylated. Conjugates are purified by chromatography or dialysis and tested by immunoassay.
Species
Label
Order No.
Amount
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IgG-HRP
(RZ ≥ 3; activity > 250 U/mg)
Modified protocol of Wilson and Nakane (1978);
molar ratio of 1 : 4 HRP K-005-HRP
K-010-HRP
K-020-HRP 5 mg
10 mg
20 mg K-005-HRP5 mgK-010-HRP10 mgK-020-HRP20 mg
(RZ ≥ 3; activity > 250 U/mg)
Modified protocol of Wilson and Nakane (1978);
molar ratio of 1 : 4 HRP K-005-HRP
K-010-HRP
K-020-HRP 5 mg
10 mg
20 mg K-005-HRP5 mgK-010-HRP10 mgK-020-HRP20 mg
IgG-AP
(recombinant, activity > 5000 U/mg)
Modified protocol of Voller and Knapp (1978);
molar ratio 1 : 4 AP K-005-AP
K-010-AP
K-020-AP 5 mg
10 mg
20 mg K-005-AP5 mgK-010-AP10 mgK-020-AP20 mg
(recombinant, activity > 5000 U/mg)
Modified protocol of Voller and Knapp (1978);
molar ratio 1 : 4 AP K-005-AP
K-010-AP
K-020-AP 5 mg
10 mg
20 mg K-005-AP5 mgK-010-AP10 mgK-020-AP20 mg
IgG-Fluoresceinisothiocyanat (Isomer I)
Modified protcol of Mohr (1991);
molar ratio 1 : 8;
determination of F/P-ratio (usually 5-8) by photometry FITC K-005-FITC
K-010-FITC
K-020-FITC 5 mg
10 mg
20 mg K-005-FITC5 mgK-010-FITC10 mgK-020-FITC20 mg
Modified protcol of Mohr (1991);
molar ratio 1 : 8;
determination of F/P-ratio (usually 5-8) by photometry FITC K-005-FITC
K-010-FITC
K-020-FITC 5 mg
10 mg
20 mg K-005-FITC5 mgK-010-FITC10 mgK-020-FITC20 mg
IgG-Biotin
Modified protocol of Wilchek and Bayer (1990);
molar ratio 1 : 50 Biotin K-005-Biotin
K-010-Biotin
K-020-Biotin 5 mg
10 mg
20 mg K-005-Biotin5 mgK-010-Biotin10 mgK-020-Biotin20 mg
Modified protocol of Wilchek and Bayer (1990);
molar ratio 1 : 50 Biotin K-005-Biotin
K-010-Biotin
K-020-Biotin 5 mg
10 mg
20 mg K-005-Biotin5 mgK-010-Biotin10 mgK-020-Biotin20 mg
All antibody conjugates are adjusted to a concentration of 1.0mg IgG/ml in SeramunStab® ST stabilizer solution.