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Purified antibodies are labeled on request either with enzymes (HRP and AP) or with fluorogens (FITC or PE). Optimized procedures guarantee preservation of immunological reactivity and enzymatic activity. Alternatively, antibodies are biotinylated. Conjugates are purified by chromatography or dialysis and tested by immunoassay.
(RZ ≥ 3; activity > 250 U/mg)
Modified protocol of Wilson and Nakane (1978);
molar ratio of 1 : 4 HRP K-005-HRP
K-020-HRP 5 mg
(recombinant, activity > 5000 U/mg)
Modified protocol of Voller and Knapp (1978);
molar ratio 1 : 4 AP K-005-AP
K-020-AP 5 mg
Modified protcol of Mohr (1991);
molar ratio 1 : 8;
determination of F/P-ratio (usually 5-8) by photometry FITC K-005-FITC
K-020-FITC 5 mg
Modified protocol of Wilchek and Bayer (1990);
molar ratio 1 : 50 Biotin K-005-Biotin
K-020-Biotin 5 mg
All antibody conjugates are adjusted to a concentration of 1.0mg IgG/ml in SeramunStab® ST stabilizer solution.