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Specific antibodies are isolated from the lgG fraction of a hyperimmune serum by immunoadsorption chromatography with insolubilized antigens. A special desorption procedure prevents loss of high affinity antibodies and keeps the antibodies fully reactive. Affinity purified antibodies are well suited as solid phase or as capture antibodies in direct and indirect solid-phase immunoassays. They are also well suited for labelling.
Affinity purified antibodies are sterile filtered and contain 0.02 % thimerosal as preservative. They do not contain other proteins as stabilizers.
Storage: 2 ... 8 °C, aliquots –20 °C
Stability: 6 months at 2 ... 8 °C, 5 years at - 20°C
Repeated freezing and thawing contributes to loss of antibody activity and should be avoided.