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Polyclonal antibodies are raised in sheep and rabbits. Balb/c mice are used for monoclonal antibody production. Non- infectious, non-toxic antigens are provided by the customers. Special immunization schedules and methods induce high affinity antibodies. For polyclonal antibodies the first ELISA tested hyperimmune serum is available 9 weeks after start of immunization.
The customer can choose between a basic package and extended service with monthly bleedings in dependence of the needed amount of hyperimmune serum. For monoclonal antibodies immunoreactivity of mouse hyperimmune sera is tested in a solid phase ELISA and one mouse is chosen for PEG mediated fusion of splenocytes with SP2/0 myeloma cells. The subsequent hybridoma screening and selection procedure follows a step by step protocol with the possibility of project termination at any step of the development.
basic (1 sheep) preimmune serum
hyperimmune serum SCHARF-001-1
extended hyperimmune serum scharf-002
basic (1 Rabbit) preimmune serum
hyperimmune serum KAN-001-1
extended hyperimmune serum kan-002
basic 1. Immunization of 3 mice
2. PEG cell fusion MAK-Maus Stufe 1
MAK-Maus Stufe 2
extended Isotype and subclass determination MAK-Subklassen