Support

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24h / 365days

We offer support for our customers

Mon - Fri 8:00am - 5:00pm (GMT +1)

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Cybersteel Inc.
376-293 City Road, Suite 600
San Francisco, CA 94102

Have any questions?
+44 1234 567 890

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info@yourdomain.com

About us

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Have any Questions? +49 33 767 - 791 10

Antibody production - Seramun services

Antibody production

Polyclonal antibodies are raised in sheep and rabbits. Balb/c mice are used for monoclonal antibody production. Non- infectious, non-toxic antigens are provided by the customers. Special immunization schedules and methods induce high affinity antibodies. For polyclonal antibodies the first ELISA tested hyperimmune serum is available 9 weeks after start of immunization.

The customer can choose between a basic package and extended service with monthly bleedings in dependence of the needed amount of hyperimmune serum. For monoclonal antibodies immunoreactivity of mouse hyperimmune sera is tested in a solid phase ELISA and one mouse is chosen for PEG mediated fusion of splenocytes with SP2/0 myeloma cells. The subsequent hybridoma screening and selection procedure follows a step by step protocol with the possibility of project termination at any step of the development.

Polyclonal antibodies

Package Antibody Order No.
Sheep
basic (1 sheep)
preimmune serum
hyperimmune serum
SCHARF-001-1

extended
hyperimmune serum scharf-002
Rabbit
basic (1 Rabbit)
preimmune serum
hyperimmune serum
KAN-001-1

extended
hyperimmune serum kan-002

Monoclonal antibodies

Package Antibody Order No.
Mouse (Balb/c)
basic
1. Immunization of 3 mice
2. PEG cell fusion
MAK-Maus Stufe 1
MAK-Maus Stufe 2
Maus (Balb/c) 3. Selection, Subcloning of 3 clones, propagation and cryoconservation MAK-Maus Stufe 3
Maus (Balb/c) 4. Transfer of selected clones or cultivation and antibody production MAK-Produktion

extended
Isotype and subclass determination MAK-Subklassen
Maus (Balb/c) IgG quantification MAK-Quantifizierung
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