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Affinity purified antibodies with proven specificity by solid phase ELISA are used for labelling. The gentle conjugation methods used, guarentee full activity of the marker and immunological reactivity of the antibodies. Labelling with highly purified horseradish peroxidase (HRP) or highly active recombinant alkaline Phosphatase (AP) results in enzyme antibody conjugates with molar ratios between 2-4 : 1 of enzyme : antibody. Labelling with fluorescein isothiocyanate isomer l (FITC) results in antibody-FITC conjugates ranging in their F/P ratios between 5 and 8 after purification.
HRP labelled antibodies are sterile filtered and contain bovine serum albumin as stabilizer. AP- and FITC- labelled antibodies contain 0.02 % sodium azide as preservative and bovine serum albumin as stabilizer.
Storage: 2 ... 8 °C, aliquots –20 °C;
Stability: 3 months at 2 ... 8 °C, 5 years at - 20°C
Repeated freezing and thawing contributes to loss of antibody activity and should be avoided.