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Cybersteel Inc.
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San Francisco, CA 94102

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Seramun News Archiv

November 2014

SeraSpot® Microarray-based multiparameter diagnostics
in microtitration plates for the medical diagnostic laboratory

07. Nov 2014 /

The product platform SeraSpot® connects the diagnostic capa­bilities of modern array techno­logies with the advan­tages of estab­lished and auto­mated ELISA tech­niques using own measure­ment solu­tions.

To detect auto­immune or infec­tious diseases, auto­antigens or proteins of patho­gens are printed in nano­liter scale in array arrange­ment on the bottom of the wells of 96well micro­titer plates. They serve as targets for anti­bodies in patient samples. Test specific controls are inte­grated into each well. The detec­tion system consists of peroxi­dase-labeled isotype-specific anti­bodies. At the site of formed immune complexes blue spots are developed. Color intensity is correlated to the concen­tration of the analyte. Pale blue to dark blue spots are visible by eye.

The test result can be read visually or by use of software-mediated image analysis with the program Seramun SpotSight®scan developed to analyze SeraSpot® assays. Image acquisi­tion is performed using proprietary readers which scan and evaluate up to 96 samples within a whole micro­titer plate. Analysis of a complete 96well plate takes only 7 min.

Video about SeraSpot®

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