Antibody purification

Polyclonal antibodies are purified from hyperimmune serum by salt precipitation followed by ion exchange chromatography (sheep) or affinity chromatography on Protein A-Sepharose (rabbit). Specific Antibodies directed to the target antigen are purified by immunoadsorption.

Monoclonal antibodies are purified from cell culture supernatant either by precipitation or ultrafiltration followed by affinity chromatography on Protein A-Sepharose or ion exchange chromatography.

Purity is checked by PAGE, reactivity by solid-phase ELISA.

2
Species Antibody Order No. Minimum amount
Sheep IgG purified by FPLC*
Affinity purified by FPLC*,**
L-021
L-023
200 mg
50 mg
Rabbit IgG purified by FPLC*
Affinity purified by FPLC*,**
L-020
L-022
25 mg
10 mg

*   antibody production to the targeted antigen is required,
** the antigen for coupling to CNBr-Sepharose 4B for affinity chromatography is provided by the purchaser

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