Antibody production

Polyclonal antibodies are raised in sheep and rabbits. Balb/c mice are used for monoclonal antibody production. Non- infectious, non-toxic antigens are provided by the customers. Special immunization schedules and methods induce high affinity antibodies. For polyclonal antibodies the first ELISA tested hyperimmune serum is available 9 weeks after start of immunization. The customer can choose between a basic package and extended service with monthly bleedings in dependence of the needed amount of hyperimmune serum. For monoclonal antibodies immunoreactivity of mouse hyperimmune sera is tested in a solid phase ELISA and one mouse is chosen for PEG mediated fusion of splenocytes with SP2/0 myeloma cells. The subsequent hybridoma screening and selection procedure follows a step by step protocol with the possibility of project termination at any step of the development.

Polyclonal antibodies

2
Package Antibody Order No.
Sheep
basic (1 sheep)

extended
 
preimmune serum
hyperimmune serum

hyperimmune serum
 
sheep-001
 
 
sheep-002
Rabbit
basic (2 rabbits)

extended
 
preimmune serum
hyperimmune serum

hyperimmune serum
 
kan-001
 
 
kan-002

Monoclonal antibodies

1
Package Antibody Order No.
Mouse (Balb/c)
basic








extended
 
 
1. Immunization of 3 mice
2. PEG cell fusion
3. Selection, Subcloning of
    3 clones, propagation and
    cryoconservation
4. Transfer of selected clones or
    cultivation and antibody
    production

    Isotype and subclass determination
    IgG quantification
 
 
MAK-Maus Stufe 1
MAK-Maus Stufe 2
MAK-Maus Stufe 3
 
 
MAK-Produktion
 
 
 
MAK-Subklassen
MAK-Quantifizierung
Service
Service

For further details

regarding product information and prices please contact us using our contact form.