Antibody conjugation

Purified antibodies are labeled on request either with enzymes (HRP and AP) or with fluorogens (FITC or PE). Optimized procedures guarantee preservation of immunological reactivity and enzymatic activity. Alternatively, antibodies are biotinylated. Conjugates are purified by chromatography or dialysis and tested by immunoassay.

4
Species Label Order No. Amount
IgG-HRP
(RZ ≥ 3; activity > 250 U/mg)
Modified protocol of Wilson and Nakane (1978);
molar ratio of 1 : 4
HRP K-005-HRP
K-010-HRP
K-020-HRP
5 mg
10 mg
20 mg
IgG-AP
(recombinant, activity > 5000 U/mg)
Modified protocol of Voller and Knapp (1978);
molar ratio 1 : 4
AP K-005-AP
K-010-AP
K-020-AP
5 mg
10 mg
20 mg
IgG-Fluoresceinisothiocyanat (Isomer I)
Modified protcol of Mohr (1991);
molar ratio 1 : 8;
determination of F/P-ratio (usually 5-8) by photometry
FITC K-005-FITC
K-010-FITC
K-020-FITC
5 mg
10 mg
20 mg
IgG-Biotin
Modified protocol of Wilchek and Bayer (1990);
molar ratio 1 : 50
Biotin K-005-Biotin
K-010-Biotin
K-020-Biotin
5 mg
10 mg
20 mg

All antibody conjugates are adjusted to a concentration of 1.0mg IgG/ml in SeramunStab® ST stabilizer solution.

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